Fig. 4. (A) Co-transfection of Δ264 CFTR and F508-del results in Golgi localization. CFBE41o^- parental cells were plated on coverslips and co-transfected with Δ264 CFTR-mCherry (red, Δ264 CFTR) and F508-des CFTR-GFP (green, F508-del). At 48 h post-transfection, the cells were fixed, permeabilized, and stained with DAPI (blue, nucleus) and antibodies against golgin-97 (purple, Golgi). The yellow signal with red arrows indicates close association or co-localization between Δ264 CFTR and Golgin-97. Representative extended-focus images are shown, and data were selected from one of four independent experiments. Co-transfection of F508-des CFTR-GFP and Δ27-264 CFTR-mCherry occurred in 21.1% of the cells (27/128); of the cells stained for golgin-97 (purple), 100% (6/6) showed co-localization or close association between Δ264 CFTR and F508-del. (B) Co-transfection of Δ27-264 CFTR and F508-del results in Golgi localization. CFBE41o^- parental cells were plated on coverslips and co-transfected with Δ27-264 CFTR-mCherry (red, Δ27-264 CFTR) and F508-del CFTR-GFP (green, F508-del). Co-transfection of F508-DEL CFTR-GFP and Δ27-264 CFTR-mCherry occurred in 23.5% of the cells (35/150); of the cells stained for golgin-97 (purple), 100% (11/11) showed co-localization or close association between Δ27-264 CFTR and F508-del. Representative extended-focus images are shown, and data were selected from one of four independent experiments.